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<title>UTas ePrints - Respiratory actions of vanilloid receptor agonists in the nucleus of the solitary tract: comparison of resiniferatoxin with non-pungent agents and anandamide</title>
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<meta content="Geraghty, D.P." name="eprints.creators_name" />
<meta content="Mazzone, S.B." name="eprints.creators_name" />
<meta content="D.Geraghty@utas.edu.au" name="eprints.creators_id" />
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<meta content="2007-10-19 03:43:23" name="eprints.datestamp" />
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<meta content="Respiratory actions of vanilloid receptor agonists in the nucleus of the solitary tract: comparison of resiniferatoxin with non-pungent agents and anandamide" name="eprints.title" />
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<meta content="Nucleus of the solitary tract; tachykinins; vanilloid; desensitization; C-®bre" name="eprints.keywords" />
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<meta content="1 Activation of vanilloid receptors on sensory nerve terminals in the commissural nucleus of the
solitary tract (cNTS) of rats with capsaicin, produces respiratory slowing. In this study, we used
microinjection techniques employing pungent and non-pungent vanilloids to further characterize
vanilloid receptors in the cNTS.
2 Microinjection of the pungent vanilloid, resiniferatoxin (RTX), into the cNTS of urethane-
anaesthetized rats, dose-dependently reduced respiratory rate without a€ecting tidal volume. RTX
was 20 fold more potent at slowing respiration (*ED50, 100 pmol) than capsaicin (*ED50, 2 nmol).
Doses of RTX greater than 100 pmol caused either irregular (dyspnoeic) breathing or terminal
apnoea (4250 pmol). The respiratory slowing response to RTX (75 pmol), was dose-dependently
attenuated by injecting RTX (but not vehicle) into the same site 60 min earlier.
3 The non-pungent phorbol derivative of RTX, phorbol 12-phenylacetete 13-acetate 20-
homovanillate (PPAHV, 0.1 - 1 nmol), also slowed respiration (ED50, *1 nmol) and almost
abolished response to RTX (75 pmol) injected into the same site 60 min later.
4 In contrast to RTX, PPAHV and capsaicin, the putative endogenous vanilloid receptor agonist,
arachidonyl ethanolamide (AEA), and non-pungent capsaicin derivative, olvanil, had no direct e€ect
on respiration. However, both AEA and olvanil dose-dependently reduced the respiratory response
to injection of RTX (75 pmol) 60 min later into the same site (EC50s, for AEA and olvanil, *2 and
0.2 nmol, respectively).
5 These studies suggest that both pungent and non-pungent vanilloids interact with vanilloid
receptors in the cNTS. However, whereas RTX and PPAHV activate and subsequently desensitize
vanilloid receptors on sensory nerve terminals in the cNTS, olvanil and AEA fail to activate despite
readily desensitizing responses to RTX in this region" name="eprints.abstract" />
<meta content="2002-11" name="eprints.date" />
<meta content="published" name="eprints.date_type" />
<meta content="British Journal of Pharmacology" name="eprints.publication" />
<meta content="137" name="eprints.volume" />
<meta content="6" name="eprints.number" />
<meta content="919-927" name="eprints.pagerange" />
<meta content="10.1038/sj.bjp.0704931" name="eprints.id_number" />
<meta content="TRUE" name="eprints.refereed" />
<meta content="0007-1188" name="eprints.issn" />
<meta content="http://dx.doi.org/10.1038/sj.bjp.0704931" name="eprints.official_url" />
<meta content="ÂCS, G., BIÂROÂ , T., A Â CS, P., MODARRES, A. & BLUMBERG, P.M.
(1997). Di€erential activation and desensitisation of sensory
neurons by resiniferatoxin. J. Neurosci., 17, 5622 - 5628.
APPENDINO, G., CRAVATTO, G., PALMISANO, G., ANNUNZIATA,
R. & SZALLASI, A. (1996). Synthesis and evaluation of phorboid
20-homovanillates: discovery of a class of ligands binding to the
vanilloid (capsaicin) receptor with di€erent degrees of coopera-
tivity. J. Med. Chem., 39, 3123 - 3131.
APPENDINO, G. & SZALLASI, A. (1997). Minireview. Euphorbium:
modern research on its active principle, resiniferatoxin, revives
an ancient medicine. Life Sci., 60, 681 - 696.
BIÂROÂ , T., MAURER, M., MODARRES, S., LEWIN, N.E., BRODIE, C.,
A Â
CS, G., A Â CS, P., PAUS, R. & BLUMBERG, P.M. (1998).
Characterisation of functional vanilloid receptors expressed by
mast cells. Blood, 91, 1332 - 1340.
BLUMBERG, P.M., SZALLASI, A. & A Â CS, G. (1993). Resiniferatoxin -
an ultrapotent capsaicin analogue. In Capsaicin in the Study of
Pain. ed. Wood, N.N. pp 45 - 82. London: Academic Press.
BRAND, L., BERMAN, E., SCHWEN, R., LOOMANS, M., JANUSZ, J.,
BOHNE, R., MADDIN, C., GARDNER, J., LAHANN, T., FARMER,
R., JONES, L., CHIABRANDO, C. & FANELLI, R. (1987). NE-
19550: a novel, orally active anti-in¯ammatory analgesic. Drugs
Exp. Clin. Res., 13, 259 - 265.
CATERINA, M.J., SCHUMACHER, M.A., TOMINAGA, M., ROSEN,
T.A., LEVINE, J.D. & JULIUS, D. (1997). The capsaicin receptor: a
heat-activated ion channel in the pain pathway. Nature, 389,
816 - 824.
DEVANE, W.A., HANUS, L., BREUER, A., PERTWEE, R.G., STEVEN-
SON, L.A., GRIFFIN, G., GIBSON, D., MANDELBAUM, A.,
ETINGER, & MECHOULAM. R. (1992). Isolation and structure
of a brain constituent that binds to the cannabinoid receptor.
Science, 258, 1946 - 1949.
DICKENSON, A.H.C., HUGHES, C., RUEFF, A. & DRAY, A. (1990). A
spinal mechanism of action is involved in the antinociception
produced by the capsaicin analogue NE 19550 (olvanil). Pain, 43,
353 - 362.
DRAY, A. & URBAN, L. (1996). New pharmacological strategies for
pain relief. Ann. Rev. Pharmacol. Toxicol., 36, 253 - 280.
GRIFFITHS, C.D., VINCENT, M.A., SZALLASI, A., COLQUHOUN,
E.Q. & GERAGHTY, D.P. (2000). Functional and desensitising
e€ects of the novel synthetic vanilloid-like agent 12-phenylacte-
tae 13-acetate 20-homovanillate (PPAHV) in the perfused rat
hindlimb. Br. J. Pharmacol., 131, 1408 - 1412.
GUO, A., VULCHANOVA, L., WANG, J., LI, X. & ELDE, R. (1999).
Immunocytochemical localization of the vanilloid receptor 1
(VR1): relationship to neuropeptides, the P2X3 purinceptor and
IB4. Eur. J. Neurosci., 11, 946 - 958.
HO, C.Y., GU, Q., LIN, Y.S. & LEE, L.Y. (2001). Sensitivity of vagal
a€erent endings to chemical irritants in the rat lung. Respir.
Physiol., 127, 113 - 124.
HUGHES, S.R., BUCKLEY, T.L. & BRAIN, S.D. (1992). Olvanil: more
potent than capsaicin at stimulating the e€erent function of
sensory nerves. Eur. J. Pharmacol., 219, 481 - 484.
British Journal of Pharmacology vol 137 (6)
926 D.P. Geraghty & S.B. Mazzone Vanilloid receptors in the brain stem
JERMAN, J.C., BROUGH, S.J., PRINJHA, R., HARRIES, M.H., DAVIS,
J.B., SMART, D. (2000). Characterization using FLIPR of rat
vanilloid receptor (rVR1) pharmacology. Br. J. Pharmacol., 130,
916 - 922.
JORDAN, D. & SPYER, K.M. (1986). Brainstem integration of
cardiovascular and pulmonary a€erent activity. In Progress in
Brain Research, Vol. 67. ed. Cervero, F. & Morrison, J.F.B.
pp. 295 - 314. New York: Elsevier Science Publishers.
KALIA, M. & MESULAM, M.M. (1980). Brain stem projections of
sensory and motor components of the vagus complex in the cat.
II. Laryngeal, tracheobronchial, pulmonary, cardiac and gastro-
intestinal branches. J. Comp. Neurol., 191, 467 - 508.
LEE, L.Y. & LUNDBERG, J.M. (1994). Capsazepine abolishes
pulmonary chemore¯exes induced by capsaicin in anesthetized
rats. J. Appl. Physiol., 76, 1848 - 1855.
LIN, Y.S. & LEE, L.Y. (2002). Stimulation of pulmonary vagal C-®bres
by anandamide in anaesthetized rats: role of vanilloid type 1
receptors. J. Physiol., 539, 947 - 955.
LIU, L., LO, Y.-C., CHEN, I.-J. & SIMON, S.A. (1997). The responses of
rat trigeminal ganglion neurons to capsaicin and to nonpungent
vanilloid receptor agonists, olvanil and glyceryl nonamide. J.
Neurosci., 17, 4101 - 4111.
LIU, L., SZALLASI, A. & SIMON, S.A. (1998). A non-pungent
resiniferatoxin analogue, phorbol 12-phenylacetate 13-actetate
20-homovanillate, reveals vanilloid receptor subtypes on rat
trigemminal ganglion neurons. Neuroscience, 84, 569 - 581.
LUKOVIC, L., DE JONG, W. & DE WIED, D. (1987). Cardiovascular
e€ects of substance P and capsaicin microinjected into the
nucleus tractus solitarii of the rat. Brain Res., 422, 312 - 318.
MATSUMOTO, S., TAKEDA, M., SAIKI, C., TAKAHASHI, T. & OJIMA,
K. (1997). E€ects of vagal and carotid chemoreceptor a€erents on
the frequency and pattern of spontaneous augmented breaths in
rabbits. Lung, 175, 175 - 186.
MAZZONE, S.B. & GERAGHTY, D.P. (1999). Respiratory action of
capsaicin microinjected into the nucleus of the solitary tract:
involvement of vanilloid and tachykinin receptors. Br. J.
Pharmacol., 127, 473 - 478.
MAZZONE, S.B. & GERAGHTY, D.P. (2000a). Respiratory actions of
tachykinins in the nucleus of the solitary tract: characterisation
of receptors using selective agonists and antagonists. Br. J.
Pharmacol., 129, 1121 - 1131.
MAZZONE, S.B. & GERAGHTY, D.P. (2000b). Respiratory actions of
tachykinins in the nucleus of the solitary tract: e€ect of neonatal
capsaicin pretreatment. Br. J. Pharmacol., 129, 1132 - 1139.
MAZZONE, S.B., HINRICHSEN, C.F. & GERAGHTY, D.P. (1998).
Hypoxia attenuates the respiratory response to microinjection of
substance P into the nucleus of the solitary tract of the rat.
Neurosci. Lett., 256, 9 - 12.
MEZEY, E., TOTH, Z.E., CORTRIGHT, D.N., ARZUBI,M.K., KRAUSE,
J.E., ELDE,R., GUO,A., BLUMBERG, P.M.&SZALLASI, A. (2000).
Distribution of mRNA for vanilloid receptor subtype 1 (VR1),
and VR1-like immunoreactivity, in the central nervous system of
the rat and human. Proc. Natl.Acad. Sci. U.S.A., 97, 3655 - 3660.
PAXINOS, G. & WATSON, C. (1986). The rat brain in stereotaxic
coordinates (2nd edition). Sydney: Academic Press Inc.
RALEVIC, V., KENDALL, D.A., JERMAN, J.C., MIDDLEMISS, D.N. &
SMART, D. (2001). Cannabinoid activation of recombinant and
endogenous vanilloid receptors. Eur. J. Pharmacol., 424, 211 -
219.
SPRAGUE, J., HARRISON, C., ROWBOTTOM, D.J., SMART, D. &
LAMBERT, D.G. (2001). Temperature-dependent activation of
recombinant rat vanilloid receptor VR1 receptors expressed in
HEK293 cells by capsaicin and anandamide. Eur. J. Pharmacol.,
423, 121 - 125.
SZALLASI, A. & BLUMBERG, P.M. (1996). Vanilloid receptor: new
insights enhance potential as a therapeutic target. Pain, 68, 195 -
208.
SZALLASI, A. & BLUMBERG, P.M. (1999). Vanilloid (capsaicin)
receptors and mechanisms. Pharmacol. Rev., 51, 159 - 211.
SZALLASI, A., BLUMBERG, P.M., ANNICELLI, L.L., KRAUSE, J.E. &
CORTRIGHT, D.N. (1999a). The cloned rat vanilloid receptor
VR1 mediates both R-type binding and C-type calcium response
in dorsal root ganglion neurons. Mol. Pharmacol., 56, 581 - 587.
SZALLASI, A., NILSSON, S., FARKAS-SZALLASI, T., BLUMBERG,
P.M., HOKFELT, T. & LUNDBERG, J.M. (1995). Vanilloid
receptors in the rat: distribution in the brain, regional di€erences
in the spinal cord, axonal transport to the periphery, and
depletion by systemic vanilloid treatment. Brain Res., 703,
175 - 183.
SZOLCSAÂ NYI, J. (1993). Actions of capsaicin on sensory receptors. In
Capsiacin in the Study of Pain. ed.Wood, J.N. pp 1 - 27. London:
Academic Press.
TUCKER, R.C., KAGAYA, M., PAGE, C.P. & SPINA, D. (2001). The
endogenous cannabinoid agonist, anandamide stimulates sen-
sory nerves in guinea-pig airways. Br. J. Pharmacol., 132, 1127 -
1135.
WAHL, P., FOGED, C., TULLIN, S. & THOMSEN, C. (2001). Iodo-
resiniferatoxin, a new potent vanilloid receptor antagonist. Mol.
Pharmacol., 59, 9 - 15.
WARDLE, K.A., RANSON, J. & SANGER, G.J. (1997). Pharmacologi-
cal characterization of the vanilloid receptor in the rat dorsal
spinal cord. Br. J. Pharmacol., 121, 1012 - 1016.
WARDLE, K.A., RANSON, J. & SANGER, G.J. (1996). Pharmacologi-
cal characterization of the vanilloid receptor in the rat isolated
vas deferens. J. Pharm. Pharmacol., 48, 285 - 291.
ZYGMUNT, P.M., PETERSSON, J., CHUANG, H., SORGARD, M., DI
MARZO, V., JULIUS, D. & HOGESTATT, E.D. (1999). Vanilloid
receptors on sensory nerves mediate the vasodilator action of
anandamide. Nature, 400, 452 - 457.
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<meta content="Geraghty, D.P. and Mazzone, S.B. (2002) Respiratory actions of vanilloid receptor agonists in the nucleus of the solitary tract: comparison of resiniferatoxin with non-pungent agents and anandamide. British Journal of Pharmacology, 137 (6). pp. 919-927. ISSN 0007-1188" name="eprints.citation" />
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<meta content="1 Activation of vanilloid receptors on sensory nerve terminals in the commissural nucleus of the
solitary tract (cNTS) of rats with capsaicin, produces respiratory slowing. In this study, we used
microinjection techniques employing pungent and non-pungent vanilloids to further characterize
vanilloid receptors in the cNTS.
2 Microinjection of the pungent vanilloid, resiniferatoxin (RTX), into the cNTS of urethane-
anaesthetized rats, dose-dependently reduced respiratory rate without a€ecting tidal volume. RTX
was 20 fold more potent at slowing respiration (*ED50, 100 pmol) than capsaicin (*ED50, 2 nmol).
Doses of RTX greater than 100 pmol caused either irregular (dyspnoeic) breathing or terminal
apnoea (4250 pmol). The respiratory slowing response to RTX (75 pmol), was dose-dependently
attenuated by injecting RTX (but not vehicle) into the same site 60 min earlier.
3 The non-pungent phorbol derivative of RTX, phorbol 12-phenylacetete 13-acetate 20-
homovanillate (PPAHV, 0.1 - 1 nmol), also slowed respiration (ED50, *1 nmol) and almost
abolished response to RTX (75 pmol) injected into the same site 60 min later.
4 In contrast to RTX, PPAHV and capsaicin, the putative endogenous vanilloid receptor agonist,
arachidonyl ethanolamide (AEA), and non-pungent capsaicin derivative, olvanil, had no direct e€ect
on respiration. However, both AEA and olvanil dose-dependently reduced the respiratory response
to injection of RTX (75 pmol) 60 min later into the same site (EC50s, for AEA and olvanil, *2 and
0.2 nmol, respectively).
5 These studies suggest that both pungent and non-pungent vanilloids interact with vanilloid
receptors in the cNTS. However, whereas RTX and PPAHV activate and subsequently desensitize
vanilloid receptors on sensory nerve terminals in the cNTS, olvanil and AEA fail to activate despite
readily desensitizing responses to RTX in this region" name="DC.description" />
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<h1 class="ep_tm_pagetitle">Respiratory actions of vanilloid receptor agonists in the nucleus of the solitary tract: comparison of resiniferatoxin with non-pungent agents and anandamide</h1>
<p style="margin-bottom: 1em" class="not_ep_block"><span class="person_name">Geraghty, D.P.</span> and <span class="person_name">Mazzone, S.B.</span> (2002) <xhtml:em>Respiratory actions of vanilloid receptor agonists in the nucleus of the solitary tract: comparison of resiniferatoxin with non-pungent agents and anandamide.</xhtml:em> British Journal of Pharmacology, 137 (6). pp. 919-927. ISSN 0007-1188</p><p style="margin-bottom: 1em" class="not_ep_block"></p><table style="margin-bottom: 1em" class="not_ep_block"><tr><td valign="top" style="text-align:center"><a href="http://eprints.utas.edu.au/2254/1/15._BJP_2002.pdf"><img alt="[img]" src="http://eprints.utas.edu.au/style/images/fileicons/application_pdf.png" class="ep_doc_icon" border="0" /></a></td><td valign="top"><a href="http://eprints.utas.edu.au/2254/1/15._BJP_2002.pdf"><span class="ep_document_citation">PDF</span></a> - Full text restricted - Requires a PDF viewer<br />235Kb</td><td><form method="get" accept-charset="utf-8" action="http://eprints.utas.edu.au/cgi/request_doc"><input accept-charset="utf-8" value="2836" name="docid" type="hidden" /><div class=""><input value="Request a copy" name="_action_null" class="ep_form_action_button" onclick="return EPJS_button_pushed( '_action_null' )" type="submit" /> </div></form></td></tr></table><p style="margin-bottom: 1em" class="not_ep_block">Official URL: <a href="http://dx.doi.org/10.1038/sj.bjp.0704931">http://dx.doi.org/10.1038/sj.bjp.0704931</a></p><div class="not_ep_block"><h2>Abstract</h2><p style="padding-bottom: 16px; text-align: left; margin: 1em auto 0em auto">1 Activation of vanilloid receptors on sensory nerve terminals in the commissural nucleus of the
solitary tract (cNTS) of rats with capsaicin, produces respiratory slowing. In this study, we used
microinjection techniques employing pungent and non-pungent vanilloids to further characterize
vanilloid receptors in the cNTS.
2 Microinjection of the pungent vanilloid, resiniferatoxin (RTX), into the cNTS of urethane-
anaesthetized rats, dose-dependently reduced respiratory rate without a€ecting tidal volume. RTX
was 20 fold more potent at slowing respiration (*ED50, 100 pmol) than capsaicin (*ED50, 2 nmol).
Doses of RTX greater than 100 pmol caused either irregular (dyspnoeic) breathing or terminal
apnoea (4250 pmol). The respiratory slowing response to RTX (75 pmol), was dose-dependently
attenuated by injecting RTX (but not vehicle) into the same site 60 min earlier.
3 The non-pungent phorbol derivative of RTX, phorbol 12-phenylacetete 13-acetate 20-
homovanillate (PPAHV, 0.1 - 1 nmol), also slowed respiration (ED50, *1 nmol) and almost
abolished response to RTX (75 pmol) injected into the same site 60 min later.
4 In contrast to RTX, PPAHV and capsaicin, the putative endogenous vanilloid receptor agonist,
arachidonyl ethanolamide (AEA), and non-pungent capsaicin derivative, olvanil, had no direct e€ect
on respiration. However, both AEA and olvanil dose-dependently reduced the respiratory response
to injection of RTX (75 pmol) 60 min later into the same site (EC50s, for AEA and olvanil, *2 and
0.2 nmol, respectively).
5 These studies suggest that both pungent and non-pungent vanilloids interact with vanilloid
receptors in the cNTS. However, whereas RTX and PPAHV activate and subsequently desensitize
vanilloid receptors on sensory nerve terminals in the cNTS, olvanil and AEA fail to activate despite
readily desensitizing responses to RTX in this region</p></div><table style="margin-bottom: 1em" cellpadding="3" class="not_ep_block" border="0"><tr><th valign="top" class="ep_row">Item Type:</th><td valign="top" class="ep_row">Article</td></tr><tr><th valign="top" class="ep_row">Additional Information:</th><td valign="top" class="ep_row">The definitive version is available at www.blackwell-synergy.com
</td></tr><tr><th valign="top" class="ep_row">Keywords:</th><td valign="top" class="ep_row">Nucleus of the solitary tract; tachykinins; vanilloid; desensitization; C-®bre</td></tr><tr><th valign="top" class="ep_row">Subjects:</th><td valign="top" class="ep_row"><a href="http://eprints.utas.edu.au/view/subjects/320502.html">320000 Medical and Health Sciences > 320500 Pharmacology and Pharmaceutical Sciences > 320502 Basic Pharmacology</a></td></tr><tr><th valign="top" class="ep_row">ID Code:</th><td valign="top" class="ep_row">2254</td></tr><tr><th valign="top" class="ep_row">Deposited By:</th><td valign="top" class="ep_row"><span class="ep_name_citation"><span class="person_name">Assoc Prof Dominic P Geraghty</span></span></td></tr><tr><th valign="top" class="ep_row">Deposited On:</th><td valign="top" class="ep_row">19 Oct 2007 14:43</td></tr><tr><th valign="top" class="ep_row">Last Modified:</th><td valign="top" class="ep_row">09 Jan 2008 02:30</td></tr><tr><th valign="top" class="ep_row">ePrint Statistics:</th><td valign="top" class="ep_row"><a target="ePrintStats" href="/es/index.php?action=show_detail_eprint;id=2254;">View statistics for this ePrint</a></td></tr></table><p align="right">Repository Staff Only: <a href="http://eprints.utas.edu.au/cgi/users/home?screen=EPrint::View&eprintid=2254">item control page</a></p>
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